Flow cytometry is a rapid method for detecting and measuring physical and chemical properties of single cells or particles in a solution. The samples are typically stained with fluorescent antibodies or dyes, or they express fluorescent proteins. The properties measured include relative size, relative granularity or internal complexity, and relative fluorescence intensity. Sorting flow cytometers allows collection of the population of interest for downstream applications.

The platform aims to become a national hub for excellence of flow cytometry by bringing together the flow cytometry units in the country. The platform includes four well established flow cytometry nodes hosted at UH/HiLIFE, UO, TAU, and UTU. Each node provides a comprehensive suite of services, including training, consultation, hands-on assistance, and 24/7 instrument access, to cater to the diverse needs of researchers from various backgrounds.

The goal is to support and facilitate high-quality research in a user-friendly and accessible manner. We also provide consultancy in experimental setup and data analysis. Contact our highly experienced staff to get going.


Contact details


Platform Chair


Nina Peitsaro
nina.peitsaro@helsinki.fi
University of Helsinki


Nodes


Node/Host UniversityNode PI
Flow cytometry unit, UH
Nina Peitsaro, UH
nina.peitsaro@helsinki.fi
TCB core, FMED, UO
Virpi Glumoff, UO
virpi.glumoff@oulu.fi
Tampere University Flow Cytometry Facility, TAU
Laura Kummola, TAU
laura.kummola@tuni.fi
CIC Cytometry, UTU
Pia Rantakari, UTU
pia.rantakari@utu.fi

TAU: Tampere University; UEF: University of Eastern Finland;
UH: University of Helsinki, UO: University of Oulu



Services


The first step on the way is to contact the platform to discuss the best set up for your experiment. Each node has well educated personnel with a broad experience in a flow cytometry.

The platform services include:

  • Open access to all nodes for flow cytometry analysis and sorting
  • Maintenance of the flow cytometers
  • Training of new users to become independent users
  • Assistance with experimental setup, panel design and data analysis
  • Flow cytometry as service run, the researcher brings the sample which is processed by the platform personnel
  • Development and setup of new methods
  • Support in analysis of flow cytometry data
  • Teaching; lectures and practical courses for undergraduate / graduate students and post-doctoral

Recent user publications


Turpin R et al.Respiratory complex I regulates dendritic cell maturation in explant model of human tumor immune microenvironment. J Immunother Cancer. 2024 Apr 11;12(4):e008053. doi: 10.1136/jitc-2023-008053.

Huuhtanen J et al. Single-cell analysis of immune recognition in chronic myeloid leukemia patients following tyrosine kinase inhibitor discontinuation. Leukemia. 2024 Jan;38(1):109-125. doi: 10.1038/s41375-023-02074-w.

Andrabi SBA et al. Long noncoding RNA LIRIL2R modulates FOXP3 levels and suppressive function of human CD4+ regulatory T cells by regulating IL2RA. Proc Natl Acad Sci U S A. 2024 Jun 4;121(23):e2315363121. doi: 10.1073/pnas.2315363121.


Danielsson et al. (2023) Nuclear lamina strain states revealed by intermolecular force biosensor. Nat Commun. 2023 Jun 30;14(1):3867. doi: 10.1038/s41467-023-39563-6.


Santaniemi et al. (2023) InfIammation and Neutrophil Oxidative Burst in a Family with NFKB1 p.R157X LOF and Sterile Necrotizing Fasciitis. J Clin Immunol. 2023 Jul;43(5):1007-1018. doi: 10.1007/s10875-023-01461-3.



Available instrumentation


For further information on the instruments, check the node´s web pages.

List of instrumentation:

Flow cytometry unit, UH

Inlux (Biomedicum)
FACSDiscover™ S8 (Biomedicum)
Accuri C6Plus
2 FACSAria II (Biomedicum and Viikki)
2 LSRFortessa (Biomedicum and Viikki)
FACSAria Fusion (Viikki)
Novocyte Quanteon (Biomedicum)
Sony SH800z (Biomedicum)

Translational cell Biology core, UO

FACSAria IIIu
LSR Fortessa
Accuri C6Plus
Discover S8
FACSsymphony A5 SE 

Tampere University Flow Cytometry Facility, TAU

BD FACSMelody
CytoFlex S 
BD FACSAria Fusion
BD FACSCanto II

CIC Cytometry, UTU

2 LSR Fortessa
2 Sony SH800 Cell Sorter
FACSymphony™ A5 SE
FACSDiscover™ S8